We are developing methods for:
axenic propagation of Sporobolus, for controlled combination with
root-associated microbes that we isolate from salt marsh sediments using in situ "microbial traps."
With these components in hand, we will populate "symcosms" with the three partners (Sporobolus, sulfate reducers, and sulfur oxidizers), to enable exploration of the symbiotic mechanisms supporting salt marsh health.
We will be actively sharing our tools and methods using the online protocols repository protocols.io.
Whether in marsh sediments, hydroponics systems, or enclosed on sterile medium, we find Sporobolus grows quickly!
"Microbial traps" designed by Ed Goluch at Northeastern provide 24 tiny wells that can be baited and placed in the environment to attract particular microbes.
We're in the design phase for the sym-systems - check back soon!